Set Appliance State:

Isolate Mains Power to appliance as per TOPs P4
CH Demand
Disconnect wires for opentherm controls and make safe
WARNING! - Mains voltage is expected during test follow
TOPs P4 live testing procedure
Mains Power On

Observation:

Does the boiler now respond to a CH demand?
Note:
If opentherm control is fitted there should be a link between the two "ROOM STAT" connections.